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Microbiology - Important Points & Tables

Important points in Microbiology

1

Father of microbiology is Anton van Leeuwenhoek 

2

Father of modern microbiology is Louis Pasteur 

3

Father of medical microbiology is Robert Koch 

4

The three division classification was proposed by Carl Woese 

5

Five kingdom classification was given by R. H. Whittaker

6

Ernst Haeckel proposed the kingdom Protista  

7

Microorganisms for the first time discovered by Anton van Leeuwenhoek

8

The first solidifying agent used in culture media is Gelatin

9

Gelatin was first discovered and used by Robert Koch 

10

Father of soil microbiology is S. Winogradsky 

11

Father of enrichment culture is Bijerinck

12

Anton van Leeuwenhoek observed the bacteria (Selenomondas) for the first time in the year 1676

13

Chemoautotropic bacteria was discovered by S. Winogardsky 

14

Lynn Margulis proposed the Endosymbiont theory

15

Peter Mitchelli proposed the chemosmotic theory 

16

Anton van Leeuwenhoek is the founder of Histology  

17

Louis Pasteur discovered the anaerobic life  

18

The term microbe was coined by Sedillot

19

The autoclave was discovered by Charles Chamberland

20

Pencillin was discovered by Alexander Flemming

21

Father of vaccination is Edward Jenner

22

The first person to find the vaccination for small pox is Edward Jenner

23

The first person to find the vaccination for anthrax is Robert Koch

24

Cell theory was proposed by Schielden and Schwann 

25

Streptomycin was discovered by Waksman

26

Endospores was discovered by Ferdinand cohn 

27

Pure culture technique for  isolation of fungi was given by Brefeld

28

Pure culture technique for isolation of bacteria was given by Robert Koch

29

First person to isolate virus is D. Iwanowasky 

30

The first person who seen protozoa under microscope is Anton van Leeuwenhoek

31

Supporter of spontaneous generation is John Needham 

32

Francessco Redi disproved the spontaneous generation theory and explained that maggots never develop from meat,  if it is covered with fine guaze  

33

Finally spontaneous generation was disproved by Pasteur

34

Tyndallisation process was developed by John Tyndall 

35

Robert hooke discovered cell in the year 1665   

36

Father of microbial techniques is Robert Koch 

37

Father of aseptic surgery is Joseph Lister 

38

The term bacterium was coined by Eherenberg  

39

Gram staining technique was developed by Hans Christian Gram 

40

The term rhizosphere was coined by Hiltner

41

Petri plates was first used to isolate microorganism is Richard J Petri

42

Azotobacter was first time isolated by Beijerinck 

43

The supporter of spontaneous generation was Aristotle 

44

Waksman got the Nobel prize in the year 1952 for the discovery of  Streptomycin 

45

Nitrogenase enzyme was first discovered by Burke in the year 1934

46

Fine structure of nif gene was given by Dr. R. Dixon

47

MLOs was discovered by Robert Koch 

48

Agar – Agar was discovered by Hesse

49

12 D concept of sterilization was given by Stumbo 

50

 L-form bacteria was discovered by Klein berger

1

Swan neck flask experiment conducted by Pasteur

2

Swan neck flask is also called as Pasteur flask or Goose neck flask   

3

Koch developed the Koch postulates which provided the guidelines to identify the causative agent of the disease

4

 In 1890, Joseph Lister insisted the importance of antisepsis in medical profession

5

John Needham observed the appearance of organisms not present at the start of the experiment and concluded that bacteria originated from the meat thus it supported the spontaneous generation theory  

6

Lazzaro Spallanzani boiled beef broth for an hour and then sealed the flasks no microbes appeared thus he disproved the spontaneous generation theory  

7

Francastro of Verona suggested that diseases might be due to invisible organisms transmitted from one to another 

8

Schleiden and Dusch passed the air through cotton in to flask containing heated broth growth did not appeared and thus disproved spontaneous generation theory 

9

Lectin (Glycoprotein) recogruition theory was proposed by Dazzo and Hubbel   

10

Diauxic groth curve was discovered by Moeod in Bacillus subtilis in the year 1945

11

Clostridium pasteurianum obligate anaerobe which is capable of fixing atomospheric nitrogen was first identified by Winogradsky   

12

Animalcules were first described by Antony Van Leeuwenhoek 

13

The word phyllosphere was given by Ruinen

14

The term mycorrhiza was coined by A. B. Frank 

15

Chemoautotrophic mode of nutrition was described by Winogradsky

16

The binomial system of nomenclature was developed by Linnaeus

17

Beijerinck isolated the root nodulating bacteria for the first time

18

Polymerase chain reaction (PCR) was discovered by Karl mullis

19

Father of plant virology Beijerinck    

20

Father of plant bacteriology E. F. smith

21

Bacteriophage was discovered by F. W. Twort and Fellix de Herelle 

22

The term viriod was coined by T. U. Diener 

23

Winogradsky discovered the several physiological distinct groups among the Autotrophic bacteria

24

Theory of evolution was given by Charles Darwin 

25

Buchner discovered the cell free alcoholic fermentation

26

The term Chromosome was given by Waldayer  

27

The term Pathogenesis was coined by Owen

28

The term Heterosis was coined by Shull in the year 1914

29

De Robertis and Franchi in the year 1953 discovered the Microtubles

30

DNA was first synthesized by A Kornberg in the year 1953

31

RNA was first synthesized by S. Ochoa in the year 1969

32

The term Protoplast was given by Purkinje

33

Berzelius suggested the term Protein

34

Buchner was the first person to discover the Enzymatic activity  

35

Funk introduced the term Vitamin 

36

Robert hook used the term cell 

37

Robert brown used the term nucleus 

38

Karl Ereky coined the term Biotechnology in the year 1919

39

Father of Genetic engineering is Paul Berg

40

Karl Mullis got the Nobel Prize in the year 1993 for the discovery of PCR technique 

41

Ruska, Luria and Anderson  were first observed the Bacteriophage 

42

Law of minimum was proposed by Liebig

43

Fraley was the first person to develop the transgenic plant in Tobacco

44

Father of modern plant pathology Anton de Barry

45

Law of heredity was given by G. J. Mendal

46

Father of genetics is  G. J. Mendal

47

Father of Nematology is Bastian 

48

Bacteria was first discovered by Anton von Leuwenhoek

49

Father of bacteriology is Anton von Leuwenhoek

50

Chromosomal theory of inheritance was proposed by Sutton and Bovery

1

Human blood groups was discovered by Karl Landsteiner in the year 1902

2

Bacterial toxins were discovered by E. von Bering and S. Kitasato in the year 1890.

3

Ernst Ruska invented the first Electron microscope in the year 1931

4

Germ theory of disease was proposed by Louis Pasteur in 1857

5

Bacterial filter were constructed by Charles Chamberland 

6

Hermatic sealing method was introduced by Lazzaro Spallanzani

7

Gene pool concept was given by Harlan and De Wet 

8

 Appertization method of sterilization was introduced by Francois Appert

9

Centers of origin concept was given by Vavilov

10

Autoclave was first designed by Charles Chamberland 

11

Millardet discovered the Bordeaux mixture

12

Lederberg introduced the term plasmid 

13

Berliner named the bacterium Bacillus thuringiensis

14

Father of Mycology is Michelie 

15

Father of Indian plant pathology E. J. Butler

16

Father of virology  Bijerinck 

17

Antifungal property of the copper sulphate was shown by P. M. A. Millardet

18

Rhizosphere effect was given by Clark. F. E.

19

Nathans and Smith were the first persons to isolate the Restriction Enzymes  

20

Reverse transcriptase enzyme was discovered by Temin and Baltimore

21

ATPase was discovered by Jens skon 

22

Peter Mitchell was proposed the Chemiosmotic model

23

Mycobacterium tuberculosis was discovered by Robert Koch   

24

Conn discovered the genus Agrobacterium 

25

Davis discovered the genus Clavibacter 

26

Clostridium genus was discovered by Pasteur 

27

Ris coined the term Genophore

28

Laversan discovered the causative agent for Malaria 

29

Glycosome term was first used by Scott and Still in the year 1968

30

Chloroplasts was discovered by Konstantin Mereschkowsky 

31

First and last person to disprove the spontaneous generation theory Francesco Redi and Louis Pasteur respectively

32

Aristotle supported the abiogenesis theory and  Anton van Leeuwenhoek supported the biogenesis theory 

33

G. E. Palade got the Nobel Prize in 1974 for the discovery of ribosomes in the year 1950 

34

The term ribosomes was coined by Richard. B. Roberts in the year 1958

35

Golgi body or golgi apparatus was discovered by Camillo Golgi in the year 1898

36

Lysosomes was discovered by Christian de Duve and Alex B. Novikoff 

37

Father of antiseptic surgery is Joseph Lister  

38

Smallest bacteria (MLOs) was discovered by Robert Koch

39

Host resistance concept was given by Vander plank 

40

Lactic acid fermentation was discovered by Louis Pasteur

41

C. N. Acharya recommended the trench method of composting

42

Dr. Rangaswamy was the first person to establish the department of agricultural microbiology in India 

43

Bangalore method of composting was developed by Dr. C. N. Acharya 

44

Abbe was the first person to use the condenser in the microscope

45

Kohler and Milstei were discovered Hybridomas

46

Berzelius was proposed the theory of Vital force  

47

Nitrification is a biological process, which was experimentally proved by Schloesing and Muntz 

48

Shepherd. R. J studied the DNA viruses in many higher plants 

49

Phagocytosis was discovered by E. Metchikoff 

50

Paul Ehrlich proposed the concept chemotherapy

1

The term gene was coined by W. L. Johannsen

2

A. V. Leeuwenhoek studied the Hanging drop method technique of microscopy to study the motility organism for the first time 

3

Lord Lister was the first person to demonstrate the antiseptic method of disease control 

4

Muller and Shank discovered the Phytoalexins 

5

M. J. Thirumalachar was the first person to use the antibiotics in plant disease control in India 

6

Epidemology of disease cycle of stem rust of wheat was first studied by Mehta. K. C.

7

Father of phytobacteriology is Erwin Fedrik smith 

8

Father of antibiotics is Alexander Flemming 

9

Burrill reported the first bacterial disease in plants

10

H. Stolp discovered the Bdellovibrio 

11

Howard and Ward were initiated the Indore method of composting

12

Peat as a carrier material in biofertilizer was first reported by V. Iswaran in India 

13

In India,  the first study of legume - rhizobium symbiosis was carried out by N. V. Joshi in the year 1920

Cross Inoculation Groups

Sl. No.

Rhizobia

Cross Inoculation Group (CIG)

1

Rhizobium leguminosarum 

Pea

2

Rhizobium trifoli

Clover

3

Rhizobium phaseoli

Bean

4

Rhizobium meliloti

Alfalfa

5

Rhizobium loti

Lotus

6

Rhizobium galegae

Soybean

7

Rhizobium fredii

8

Rhizobium sp.

Chick pea

9

Rhizobium sp.

Cow pea

Different types of Biofertilizers

Sl. No.

Groups

Examples

 N2  fixing Biofertilizers

1

 Free-living

Azotobacter, Clostridium,  Anabaena, Nostoc,

2

Symbiotic

Rhizobium, Frankia, Anabaena azollae

3

Associative Symbiotic

Azospirillum

P Solubilizing Biofertilizers

1

Bacteria

 Bacillus megaterium var. phosphaticum, Pseudomonas striata

2

Fungi

Penicillium sp, Aspergillus awamori

P Mobilizing Biofertilizers

1

Arbuscular mycorrhiza

Glomus sp., Gigaspora sp., Acaulospora sp.,

2

Ectomycorrhiza

Laccaria sp., Pisolithus sp., Boletus sp., Amanita sp.

3

Orchid mycorrhiza

Rhizoctonia solani

Plant Growth Promoting Rhizobacteria

1

 Pseudomonas

Pseudomonas fluorescens

Biofertilizers for Micronutrients

1

Silicate and Zinc solubilizers

Bacillus sp.

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